ABSTRACT

The study was preformed on 4 groups of male Wistar rats receiving p.o through 3 months every day: 1) sodium nitrite in dose 30mg/kg b.w. x day (0,2 LD₅₀);2) copper chloride in dose 4.67 mg/kg b.w. x day (0.03 LD₅₀);3) copper chloride and sodium nitrile in amounts as above and 4 – control group – received dest. Water. The methemoglobin were determined in whole blood and tryptophan in plasma 24 hours after the last intoxication. There was showed, that every day intoxication of rats with sodium nitrite cause the increase of methemoglobin concentration and decrease the free tryptophan leven in the blood. There was also observed, that copper chloride, administrated together with sodium nitrite, decreases significantly his methemoglobincreative action.